Effects of dietary glutathione on the growth performance, skin mucus antioxidant capacity, and immune responses of juvenile taimen Hucho taimen

A 49-day feeding trial was conducted to examine the effects of dietary glutathione on growth performance, skin antioxidant capacity, immune responses, and gene expression in juvenile taimen (Hucho taimen). The experimental fish had a mean initial weight 5.36 ± 0.13 g were fed diets (approximately crude protein 460 g/kg, gross energy 22 MJ/kg) containing graded amounts (0–control, 100, 200, 400, 800 mg/kg). Each group 3 replicates with 500 per tank (500 L). Fish four times daily until evident satiation. After trial, 60 from each challenged lipopolysaccharide for 7 days, mucus antibacterial activity, ability, also determined. gain (WG, 3642.33–3843.67 g) survival rate (SR, 93.07–94.19%) all significantly improved (100–800 mg/kg) compared control 3414.30 g; SR, 90.61%) (P < 0.05). 7-day (3.00 mg/kg body weight) challenge, group, increased protein, lysozyme activity (LZM, 2.67–2.74 U/mg; control, 2.11 U/mg), alkaline phosphate (ALP, 5.09–5.34 U/L; 4.50 U/L), minimal inhibitory concentration (MIC, 100–200 μL/mL; > 200 μL/mL), antimicrobial (7.72–9.11 mm; 7.19 mm) Furthermore, capacity superoxide dismutase (SOD, 9.45–9.52 7.51 peroxidase (GSH-px, 9.68–10.11 8.68 U/L) higher 0.05), whereas malondialdehyde (MDA, 0.18–0.20 nmol/L; 0.23 nmol/L) lower Additionally, decreased levels tumor necrosis factor (TNF-α), interleukin 1β (IL-1β), 6 (IL-6), 8 (IL-8), but 10 (IL-10) nuclear factor-kappa-B inhibitor alpha-like (IκBα). In conclusion, performance lipopolysaccharide-induced inflammatory response, indicating that has potential anti-inflammatory H. taimen.